Teknik Tes Hemoglobin

Oleh :

Bianda Pramudita MSc

Teknik tes hemoglobin mengukur konsentrasi hemoglobin, dimana pengambilan sampel darah dilakukan dengan menusukkan jarum ke vena, biasanya di lengan, dan darah diambil dalam tabung. Sampel darah kemudian dimasukkan dalam larutan yang menghancurkan sel darah merah dan melepaskan hemoglobin.

Hemoglobin bereaksi dengan zat kimia yang menghasilkan warna, dan absorbansi cahaya diukur menggunakan spektrofotometer. Hasilnya dinyatakan dalam satuan konsentrasi hemoglobin seperti gram per desiliter (g/dL) atau milimol per liter (mmol/L).[2,3]

Persiapan Pasien

Referensi

2. Whitehead RD Jr, Mei Z, Mapango C, Jefferds MED. Methods and analyzers for hemoglobin measurement in clinical laboratories and field settings. Ann N Y Acad Sci. 2019 Aug;1450(1):147-171. doi: 10.1111/nyas.14124. Epub 2019 Jun 4. PMID: 31162693; PMCID: PMC6709845. Whitehead RD Jr, Mei Z, Mapango C, Jefferds MED. Methods and analyzers for hemoglobin measurement in clinical laboratories and field settings. Ann N Y Acad Sci. 2019 Aug;1450(1):147-171. doi: 10.1111/nyas.14124. Epub 2019 Jun 4. PMID: 31162693; PMCID: PMC6709845
3. Quinn JG, Tansey EA, Johnson CD, Roe SM, Montgomery LE. Blood: tests used to assess the physiological and immunological properties of blood. Adv Physiol Educ. 2016 Jun;40(2):165-75. doi: 10.1152/advan.00079.2015. PMID: 27068991.
11. Srikanth KK, Lotfollahzadeh S. Phlebotomy. In: StatPearls. Treasure Island (FL): StatPearls Publishing; 2023 [cited 2023 Sep 15]. http://www.ncbi.nlm.nih.gov/books/NBK574569/
12. Royal JT, Fisher JT, Mlinar T, Mekjavic IB, McDonnell AC. Validity and reliability of capillary vs. Venous blood for the assessment of haemoglobin mass and intravascular volumes. Front Physiol. 2022 Nov 24;13:1021588. doi: 10.3389/fphys.2022.1021588. PMID: 36505074; PMCID: PMC9730879.
13. Boghani S, Mei Z, Perry GS, Brittenham GM, Cogswell ME. Accuracy of Capillary Hemoglobin Measurements for the Detection of Anemia among U.S. Low-Income Toddlers and Pregnant Women. Nutrients. 2017 Mar 9;9(3):253. doi: 10.3390/nu9030253. PMID: 28282926; PMCID: PMC5372916.
14. Cappellini MD, Motta I. Anemia in Clinical Practice-Definition and Classification: Does Hemoglobin Change With Aging? Semin Hematol. 2015 Oct;52(4):261-9. doi: 10.1053/j.seminhematol.2015.07.006. Epub 2015 Jul 17. PMID: 26404438.
15. Garcia-Casal MN, Pasricha SR, Sharma AJ, Peña-Rosas JP. Use and interpretation of hemoglobin concentrations for assessing anemia status in individuals and populations: results from a WHO technical meeting. Ann N Y Acad Sci. 2019 Aug;1450(1):5-14. doi: 10.1111/nyas.14090. Epub 2019 Apr 21. PMID: 31006883; PMCID: PMC6703163.
17. Karakochuk CD, Hess SY, Moorthy D, Namaste S, Parker ME, Rappaport AI, Wegmüller R, Dary O; HEmoglobin MEasurement (HEME) Working Group. Measurement and interpretation of hemoglobin concentration in clinical and field settings: a narrative review. Ann N Y Acad Sci. 2019 Aug;1450(1):126-146. doi: 10.1111/nyas.14003. Epub 2019 Jan 16. PMID: 30652320.
18. Bansal PG, Toteja GS, Bhatia N, Gupta S, Kaur M, Adhikari T, Garg AK. Comparison of haemoglobin estimates using direct & indirect cyanmethaemoglobin methods. Indian J Med Res. 2016 Oct;144(4):566-571. doi: 10.4103/0971-5916.200882. PMID: 28256465; PMCID: PMC5345303.
19. Toppo M, Pal DK, Gour D, Melwani V, Dubey M, Mishra A. Comparison of Performance of Digital Hemoglobinometer over Automated Hematology Analyzer for Hemoglobin Estimation and Its user-friendliness among the Pregnant Women in Selected District Hospitals of Madhya Pradesh. Indian J Community Med. 2019 Jan-Mar;44(1):31-34. doi: 10.4103/ijcm.IJCM_216_18. PMID: 30983710; PMCID: PMC6437795.

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